FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002295978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002295978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388875
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC209040.8750562503270368No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC135350.5665846894458689No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC100450.4204908168070744No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC93430.39110459944534565No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG89450.37444403746533406TruSeq Adapter, Index 7 (96% over 28bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC84620.35422531526346085No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC66410.2779969650986343No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCA50760.2124849563078855No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG35530.14873109727382136RNA PCR Primer, Index 7 (96% over 28bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC35400.14818690806341897No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC33710.14111244832818795No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG33240.13914499502904087No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGAC30620.12817748940400817No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGA27900.11679137669405054No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC25770.10787504578514992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)