FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002296005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262823
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG111660.3422189925717699RNA PCR Primer, Index 26 (100% over 29bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG48860.14974762651850868No Hit
GAGCTGAGGTGAAGATCCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT40400.12381915905337187No Hit
GTATCAACGCAGAGTACATGGGGGATCTTCACCTCAGCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACT34460.10561406487572264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)