FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGTCGCGGGTCGGAGCTCGGTCCTGCTGGAGGCCACGGGTGCCACACACTCGGTCCCGACATGATG13.225806451612903No Hit
ACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCCTGTCTCTTATACACATCT13.225806451612903No Hit
CCCAATGGATGACAAAAAGAAGAAACGGAGTCCCAAGCCCTGCCTGGCCCAGCCAGCCCAGGCCCCAGGCACACC13.225806451612903No Hit
GCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAAATCTAGAGTAAAACCAAGCC13.225806451612903No Hit
ACACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGCTGTCT13.225806451612903No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG13.225806451612903RNA PCR Primer, Index 40 (96% over 30bp)
GGGTGCGGGTGCAGATAAGCCAAAAGGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGGC13.225806451612903No Hit
GTATCAACGCAGAGTACATGGGAGGCTTTCAAGATCCAGACTATATATCACCTCTCACATAAACACATCACAGAG13.225806451612903No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGAAGATAGACACAGTCTCTGTTACTTTATATTTTACAAATATCCGAG13.225806451612903No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13.225806451612903Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACATGGGCGTGTCCCACTTCACGCCTCTCTCATTTAGACAAAACCCAGCGCAGCGCCCACCA13.225806451612903No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTCCGAGCCCACGAG13.225806451612903No Hit
GGTATCAACGCAGAGTACATGGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTA13.225806451612903No Hit
AAACATCTCAGAGCACAAGAGACCGATCACAACCTCGTATGGCGACATATGCTTGAAAAAAAAACGAATTGGGTT13.225806451612903No Hit
CTGCGTCTCTGTGCGGCTGCCATTGGCATAGAGCGTTTCATAAGACAGATATTGCTTGGCCTCTTCGTAAGTGGG13.225806451612903No Hit
CTCCTGGCCACAGGGGGGCCCCTGAGGTTGCAGGTCTGGGACTGTCTCTTATACACATCTCCGAGCCCACGAGAC13.225806451612903No Hit
GTATCAAAAAGCTTTATTTCCATTTGGTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGG13.225806451612903No Hit
CATGGGAGGTTTACAAGTTCCAGACTATCTCTGTGTGGAATTGTGGTGAGATTTTGTGTAAAAATGAATGGCATC13.225806451612903No Hit
ATCTGGGCTCATTGCAACCTCTGCCTCCCGGGTCCAAGCGATTCCCCTGTCTCAGCCTCCCGAGTAGGTGGGATT13.225806451612903No Hit
GTCTCGGACTGTAGCGAGGGGCTCTTGGGGCTGTATACTGCCCTGCCGTTCAGGAACTCCTCCGATGCCTGGCAG13.225806451612903No Hit
ACCACATGGCATCGTAGGCTGCTCTGTCAAAGGATATAGCGATAGCTGGTCCTATGGGCAGATGCCTAGAATCAA13.225806451612903No Hit
AACGATTTCTCACCAAATCACTACACAGGACAGCAAAGGGGTGAGAAGGGGCTGAGGGAGGAAAAGCCAGGAAAC13.225806451612903No Hit
GTCAGTGACCTGGTCTCCTAATCAGCTTCCTAAAGGCCTTTGACATGTGGAGATCATGGCCCCCCTACTTCGGAT13.225806451612903No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTAAGCGGGCAACCGCGCGCAGGTTTT13.225806451612903No Hit
CAGCGGGCGCAGCTGCACGAAGGGCACAAAGTCCGGCGCCACGGCGGGCTCCCGCCGCAACCGCCGCAGCAGCCG13.225806451612903No Hit
TGGCATCGTCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACAACGAGCTGCGCGTGGCCCC13.225806451612903No Hit
CATCAACGCCCTGTGCTTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGA13.225806451612903No Hit
GTCTACCAGCGGGTCTGGCTACTGTTGCTGTGGATTCCTGGGCAATACCAATCCTGCTCAACGCTTTGGATGTCC13.225806451612903No Hit
GTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13.225806451612903No Hit
GAGCAGAACTGTGTCGCTCCGCGCATCTCCTTTGCTGCGGTCCAGCCGCGTCTCACTGCCCAACCCTGGGGCCAT13.225806451612903No Hit
GCGTGAACCCCGATGTGGCGCAGAGCGGCAGCACCCAGCAGACCGGCAGCTCCGGCCGCACCGGCCTGCAGTGGG13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)