FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002296023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466285
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630494.299914409545211No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCG69300.47262298939155756No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG54780.3735972201857074No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG52230.3562063309656718No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTAA50580.3449534026468251No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA48730.3323364830166032No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCC46450.31678698206692424No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC43670.29782750283880693No Hit
ACACAATGCCATTCATTTTTACACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCA40690.27750403229931425No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC39120.2667967005050178No Hit
CACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTG38020.25929474829245336No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACTCC38020.25929474829245336No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGACTCC35790.24408624517061828No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTA35250.2404034686299048No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCTG35130.23958507384307962No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC34610.2360386964335037No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA34490.2352203016466785No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT33930.23140112597482754No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCT31570.21530602850059846No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA31520.2149650306727546No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCT30880.2106002584763535No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACAT30470.20780407628803402No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGC28420.19382316534643673No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGACT27570.18802620227309153No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT26990.184070627470103No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACT26490.18066064919166464No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGACTC26310.17943305701142684No Hit
CCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC25320.17268130002011886No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACAT25260.17227210262670628No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGACTC25230.17206750392999998No Hit
CACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCT25030.1707035126186246No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACAC24390.1663387404222235No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGC23170.15801839342283389No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC22550.15379002035757033No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCT22330.15228962991505746No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC21390.1458788707515933No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGC20690.1411049011617796No Hit
TTTTTACACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGA20620.14062750420279824No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT20210.13783132201447879Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGACTGTCTCTTA19960.13612633287525958No Hit
TCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTA19910.13578533504741575No Hit
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGA19870.1355125367851407No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTGC19750.13469414199831548No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC19690.13428494460490287No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGAC19120.13039756936748312No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTCTGTCTC19060.12998837197407054No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAC18710.12760138717916367No Hit
CAATTGACGTTACCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTT18100.12344121367946886No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC17890.12200902280252474No Hit
CCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACA17360.11839444582738008No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGA16960.1156664632046294No Hit
TATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGG16290.11109709231152197No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT16220.1106196953525406Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACA15900.10843730925434004No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTT15430.10523192967260799No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGACGACTCCT15310.10441353488578277No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTCT15290.10427713575464524No Hit
CTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTT15210.10373153923009511No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACG15130.10318594270554496No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCC14910.10168555226303208Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGT14900.10161735269746332No Hit
ACACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCT14760.10066255877950057No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTGACGCTGCCG14740.10052615964836305No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACG14680.10011696225495044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)