FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55083
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA33236.032714267559864No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG31415.702303796089538No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG31095.64420964725959No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT29465.348292576657045No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC27885.061452716809179No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT27715.030590200243269No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG24894.518635513679357No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT23514.268104496850208No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC23164.204564021567453No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC22544.092006608209429No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG21633.926801372474266No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA16422.9809560118366827No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG16332.96461703247826No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT16022.908338325799248No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG15952.895630230742697No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT15522.8175662182524555No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG15082.737686763611278No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG10421.8916907212751668No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG10311.871720857614872No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA9871.7918414029736942No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT9731.7664252128605922No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT8281.5031861009748924No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG8131.4759544687108546No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT7901.4341992992393298No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC7011.272624947806038No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC5330.9676306664488136No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT5130.9313218234300964No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC5060.9186137283735454No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT4420.8024254307136504No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA4410.8006099885627144No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT2870.5210318973185919No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT2120.3848737359984024No Hit
AGCCAAAGGGTCTAAAGAAGTGGAAGTCGCTCTTGTTCGGAAAGACAAAG830.1506816985276764No Hit
AGCCAAAGGGTCTAAAGAAGTGGAAGTCACTCTTGTTCGGAAAGACAAAG800.14523537207486883No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)