FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002296098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452941
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT88061.944182575655549No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT81951.809286419202501No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG50931.124429009517796No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC45110.9959354529618648No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG39900.8809094341205588No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC33570.7411561329179739No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA31070.6859613062186907No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT30400.6711690926632828No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT26480.5846236043988069No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT24680.544883329175323No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCC24240.5351690396762492No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG22590.49874045405472234No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT20600.454805372002093Illumina Paired End PCR Primer 2 (95% over 22bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG20490.4523767996273245No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT20080.44332484804864214No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC18650.41175340717665215No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT18370.4055715865863324No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA17990.3971819729280414No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT17400.3841559938270106No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT16480.3638442976016744No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT15710.3468442909782952No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTT14150.31240271911794254No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC13920.3073247950616085No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC12940.28568842299548947No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA12610.2784027058711841No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC11770.259857244100225No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT11640.25698711311186223No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT11260.2485974994535712No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT11210.24749360291958555No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT10920.2410910030224687No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT10770.2377793134205117Illumina Paired End PCR Primer 2 (95% over 24bp)
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT10670.2355715203525404No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT10450.23071437560300348No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC9940.2194546309563497No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCT9910.21879229303595835RNA PCR Primer, Index 1 (95% over 21bp)
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC9690.2139351482864214No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC9570.2112857966048558No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT9320.2057663139349275No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA8670.19141565899311389No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA8620.19031176245912823No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA8350.18435072117560566No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT8240.1819221488008372No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC8190.18081825226685155No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA8160.18015591434646014No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT7980.17618188682411176No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT7410.1635974663366752No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT7210.15918188020073254No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGGCGT7080.15631174921236982No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA6900.15233772169002144No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG6860.1514546044628329No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG6580.1452727838725132No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG6560.14483122525891895No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC6020.13290914269187376No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTATGCCGT5950.13136368754429384No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA5870.12959745308991677No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA5860.12937667378311965No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5850.12915589447632253No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCT5840.1289351151695254No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTTTGCCGT5790.12783121863553973No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGC5760.1271688807151483No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT5760.1271688807151483No Hit
GCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCCTATTGGGA5670.12518186695397415No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC5540.1223117359656114No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT5320.1174545912160745No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCT5310.11723381190927736No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC5190.11458446022771178No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATG4970.10972731547817485No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC4940.10906497755778345No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA4930.10884419825098633No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTT4920.1086234189441892No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCG4790.10575328795582647No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGG4770.10531172934223221No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA4740.1046493914218408No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT4710.1039870535014494No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCTGTCTC4600.10155848112668095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)