FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002296114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346049
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT194625.624059020543334No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG111303.2163075171435263No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA94612.734005877780314No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA94602.73371690136368No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG81632.3589144889885536No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG76062.19795462492306No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC69702.01416562394343No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG68371.975731760531023No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC60511.7485962970561972No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT53431.5440009940788733RNA PCR Primer, Index 29 (95% over 23bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC33840.9778961938916164No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA33200.9594017032269997No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT30080.8692410612369925No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT29770.8602827923213187No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT26660.7704111267479461RNA PCR Primer, Index 29 (96% over 25bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT26450.7643426219986187No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT23100.6675355224260148No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT21610.6244780363474537No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT21070.6088733098491832No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC18870.5452984981895628No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT17680.5109103046100407No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT16490.4765221110305188No Hit
AAAGTTGAAATGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCG15870.4586055731991712No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT14340.41439218145407153No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA14240.4115024172877252No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT14050.406011865371667No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT13740.39705359645599325No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC13670.39503076153955075No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT13500.3901181624567619No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC12950.3742244595418568No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT12230.35341815754416284No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG11810.341281148045508No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT11710.3383913838791616No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT11520.3329008319631035No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGCCGT11480.33174492629656493No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC11010.31816303471473695No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC10510.303714213883005No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC10120.2924441336342541No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9730.2811740533855032No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT9650.2788622420524261No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC9530.2753945250528105No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT9440.2727937373030987No Hit
AAAGTTGAAATGTCAGGGTCATGTCCTTCTTGATCTGTCTCTTATACACA9180.2652803504705981No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC8990.25978979855454No Hit
AAAGTTGAAATATCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA8910.25747798722146287No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA8790.2540102702218472No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC8730.2522764117220394No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAAT8380.242162237139827No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC8150.23551577955723035No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA8000.23118113330771076No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGT8000.23118113330771076No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTA7580.21904412380905594No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG7240.20921892564347822No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCT7110.20546223222722795No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTG7040.20343939731078545Illumina PCR Primer Index 10 (96% over 25bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCT6850.19794884539472732No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT6790.1962149868949195No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC6610.19101341139549602No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT6550.18927955289568818No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA6520.18841262364578426No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG6350.1835000245629954No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT6340.18321104814636077No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA6310.18234411889645688No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA6300.18205514247982224No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG6270.1811882132299183No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC6100.17627561414712947No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAG5900.1704960858144367No Hit
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCT5900.1704960858144367No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACC5880.1699181329811674No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATAC5800.1676063216480903No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT5580.16124884048212823No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA5580.16124884048212823No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGG5570.16095986406549362No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT5380.1554693121494355No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC5270.15229057156645445No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA5260.1520015951498198No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC5150.1488228545668388No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACAC5030.14535513756722312No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA4980.14391025548404995No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC4970.14362127906741531No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT4970.14362127906741531No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTC4910.14188742056760748No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTA4880.14102049131770356No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC4860.14044253848443428No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCA4610.13321812806856834No Hit
GATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACA4550.13148426956876047No Hit
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGA4550.13148426956876047No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATTCC4370.126282694069337No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGC4350.1257047412360677No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT4310.12454883556952917No Hit
GTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATC4250.12281497706972135No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAG4180.12079214215327888No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCTGTCTC4120.11905828365347104No Hit
GTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTG4090.11819135440356712No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTC4090.11819135440356712No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATTCCGT4060.11732442515366322No Hit
CATCCATAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAG4030.11645749590375928No Hit
TTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTA4010.11587954307049002No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT4000.11559056665385538RNA PCR Primer, Index 29 (95% over 22bp)
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCT4000.11559056665385538No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT3960.11443466098731683No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCTGTCTCTTATA3940.11385670815404754No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTG3900.112700802487509No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT3900.112700802487509No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT3800.10981103832116261No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGT3800.10981103832116261No Hit
CATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAA3750.10836615623798942No Hit
CATCCGCAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAG3740.10807717982135479No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCCT3710.10721025057145087No Hit
CGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC3710.10721025057145087No Hit
CTGCTTCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCT3690.10663229773818159No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTGT3680.10634332132154695RNA PCR Primer, Index 29 (95% over 23bp)
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT3630.10489843923837375No Hit
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGA3580.10345355715520056No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGGCGT3560.1028756043219313No Hit
GTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA3500.10114174582212346No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG3500.10114174582212346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)