FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181657
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT1886410.384405775720177No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC61093.362931238542968No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT58363.2126480124630485RNA PCR Primer, Index 11 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT51962.860335687587046TruSeq Adapter, Index 11 (95% over 23bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG45212.488756282444387No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG27621.5204478770429986No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG27541.5160439729820487No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT27311.5033827488068172No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG24871.3690636749478413No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT23851.3129138981707285No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC20501.1285004156184457No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA18591.0233572061632639No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG18431.0145493980413636TruSeq Adapter, Index 11 (96% over 26bp)
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT17660.9721618214547195No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT16760.9226179007690318No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT16050.8835332522281003No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT13430.7393053942319866No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA11870.653429265043461No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTT11550.6358136487996608No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC10970.6038853443577731No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT10860.5978299762739668No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT10660.5868202161215917No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC10390.5719570399158854No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT10110.5565433757025604No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT9160.5042470149787787No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG8040.4425923581254782No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT7990.43983991808738443No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC7770.42772918191977194No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT7410.4079116136454967No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT6860.37763477322646527No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA6730.37047842912742146No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACAT6660.3666250130740902No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC6260.34460549276934No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT6160.3391006126931525No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTAC6040.33249475660172745No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA5700.3137781643426898No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTAC5540.3049703562207897No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC5520.3038693802055522No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC5330.29341010806079587No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC5050.2779964438474708No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT4950.27249156377128325No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTA4860.26753717170271446No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA4750.26148180361890816No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT4720.2598303395960519No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT4680.2576283875655769No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC4430.243866187375108No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA4330.23836130729892052No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC4300.23670984327606423No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACAT4200.23120496319987668No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCTGTCTC4200.23120496319987668No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT4120.22680105913892665No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT4010.22074569105512035No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA3970.21854373902464533No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC3860.21248837094083906No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG3800.20918544289512653No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA3760.2069834908646515No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTC3660.20147861078846396No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA3560.19597373071227642No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT3490.19212031465894516No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT3470.19101933864370765No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT3400.18716592259037637No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGC3340.18386299454466384No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTGT3320.18276201852942633Illumina PCR Primer Index 11 (95% over 24bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC3270.18000957849133256No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA3180.17505518642276377No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC3040.1673483543161012No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA2910.16019201021705742No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAG2830.1557881061561074No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG2810.15468713014086988No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA2780.1530356661180136No Hit
TACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC2750.15138420209515738TruSeq Adapter, Index 11 (95% over 24bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC2740.1508337140875386No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTA2710.14918225006468233No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCT2710.14918225006468233No Hit
CAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC2700.1486317620570636No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA2700.1486317620570636No Hit
GTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA2640.14532883401135105No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT2610.1436773699884948No Hit
CATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACA2580.14202590596563855No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT2570.14147541795801977No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT2500.1376220019046885No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG2490.13707151389706976No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT2480.136521025889451No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC2440.13431907385897598No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT2400.13211712182850097No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG2400.13211712182850097No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA2370.1304656578056447No Hit
CATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC2330.12826370577516968No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCTGTCTCTTATA2230.12275882569898215No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG2210.12165784968374464No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG2170.11945589765326961No Hit
GCGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCT2160.11890540964565087No Hit
CGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA2130.1172539456227946No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCTGTCTCTTATACACATC2100.11560248159993834No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT2030.11174906554660706No Hit
GGTCACGTCCTTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG2030.11174906554660706No Hit
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCT2020.11119857753898832No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGC1990.10954711351613206No Hit
CAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTA1970.10844613750089455No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCCTGTCTCT1960.10789564949327578No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC1960.10789564949327578No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT1930.10624418547041953RNA PCR Primer, Index 11 (95% over 22bp)
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGCTGTCTCTTATACACA1890.10404223343994451No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAG1880.10349174543232575No Hit
CATCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATC1880.10349174543232575No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACAT1870.102941257424707No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCCTGTCTCTTATACA1850.10184028140946949No Hit
GGTCACGTCCTTCTTGATCCTGTCTCTTATACACATCTCCGAGCCCACGA1840.10128979340185074No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT1840.10128979340185074No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATA1820.10018881738661323No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)