FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002296149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAAACTGTGGGAGCAGGACTAGACTCTTCTGGCAGGCAGAGAGCTACTCCTCGGCCAGTGTTCGAGCCTGGTC110.0No Hit
TCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG110.0Illumina PCR Primer Index 10 (96% over 27bp)
CTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG110.0Illumina PCR Primer Index 6 (96% over 27bp)
CTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG110.0RNA PCR Primer, Index 1 (96% over 28bp)
AAAAAGACTAAATTTTGAAACAATTAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAT110.0No Hit
GACAGTCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG110.0RNA PCR Primer, Index 47 (96% over 30bp)
TCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG110.0TruSeq Adapter, Index 6 (96% over 28bp)
TCTCCGAGCCCACGAGACCAATCCTCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG110.0TruSeq Adapter, Index 7 (96% over 26bp)
GCACGACACCGAAGCGGTGGGAGCAGAAATGCTCGGTAGGAGAGCGTTGCAGTCGCGGCGAAGGAGAACGGACGC110.0No Hit
CATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG110.0Illumina PCR Primer Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)