FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002296150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG211.76470588235294Illumina PCR Primer Index 6 (96% over 27bp)
CAACCATAGCGTCCTAAGCTGTCTCTTATACACATCTCCGAGCCCACGGACATAAACACATATCGTATTCCGTCT15.88235294117647No Hit
CTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG15.88235294117647TruSeq Adapter, Index 18 (96% over 29bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGACTGTCTCTTATACACATCTCCGAGCCCACG15.88235294117647No Hit
GCTCCCATGTTCTCCCTCCCTCCCATTGTAGCCTTGCTGCCCAGGGGAGGGATATGTCTTCCTTTATGCATCTGG15.88235294117647No Hit
TCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.88235294117647TruSeq Adapter, Index 6 (96% over 28bp)
CTCCGAGCCCACGAGACGTTGGAAGATGTCGTATGCCGTCTTCTGCTTGAAAAATAAATGGGGGGGGGGGGGGGG15.88235294117647RNA PCR Primer, Index 9 (96% over 26bp)
GCACCGGCGCCTGGACATCATCGTGGTGCCCTATAGCGAGTTTGCCTGTGCCCTGCTCTACTTCACCGGCTCTGC15.88235294117647No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCC15.88235294117647No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACATTCTTTTAAATCCTCAAGAATTAAGCAAAAAGCAGCAA15.88235294117647No Hit
CTCATACTAGCCCCCGTGAACCCCCGCACAGCTAAGGGCATCACAGACCTGATATTGATCAATCTCGGGAGGCAC15.88235294117647No Hit
CTGCGTGGCAGTCCTGATTTCCATGCTTCTGGAGAATCCATTTCGTTAACACTGAAAGCCAGTTCTCTTTTCCTG15.88235294117647No Hit
TCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG15.88235294117647Illumina PCR Primer Index 7 (96% over 27bp)
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAGAAGCAAACGCACGGGTAAGCGG15.88235294117647No Hit
CATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG15.88235294117647Illumina PCR Primer Index 10 (96% over 27bp)
CCCGATGAGCAGCCACTACCTGTCTCTTATACACATCTCCGAGCCCACGGACATCCTCTCATATCGTATGCCGTA15.88235294117647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)