FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002296177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006955
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGC224942.233863479500077No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC110311.0954809301309394No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC101881.0117631870341772No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC91290.9065946343183161No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG85110.8452214845747824TruSeq Adapter, Index 4 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGC84460.8387663798282942No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC69030.6855321240770442No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAA52600.5223669379465815No Hit
ACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG37000.36744442403086536RNA PCR Primer, Index 4 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAAC34070.33834679801977247No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG33070.3284158676405599RNA PCR Primer, Index 4 (96% over 28bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC31820.31600220466654416No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAT29730.29524656017398987No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG28010.2781653599217443No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAA27260.2707171621373349No Hit
ATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG25550.25373527118888134TruSeq Adapter, Index 4 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC21510.2136143124568625No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAAT21370.21222398220377275No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC21310.21162812638102No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAT17950.17826020030686573No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAAT17150.17031545600349568No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAA17100.16981890948453507No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATG15940.15829903024464848No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG15090.14985773942231778No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACA15010.14906326499198078No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC14730.14628260448580124No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACA14130.14032404625827372No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACA14040.13943026252414456No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG12050.11966771106951156No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA11810.11728428777850054No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAT11450.113709152841984No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAA11060.10983608999409109No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11000.10924023417133834No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACA10860.10784990391824857No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAAT10490.10417545967793994No Hit
CACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG10420.10348029455139504RNA PCR Primer, Index 36 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)