FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838771
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC1178196.407486304711135No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC704413.8308739913779366No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC617483.3581125654037396No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC490682.6685215287819966No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC402472.188798931460198No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC391532.129302670098669No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCA186831.016059095994009No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC159130.8654149972998268No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCT115160.6262878846794951No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG97210.528668333359619Illumina PCR Primer Index 7 (96% over 27bp)
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGC73070.39738499247595266Illumina PCR Primer Index 7 (95% over 24bp)
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTT42230.22966427031968634No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATC41180.22395393444860726No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC41150.22379078199514785No Hit
GTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC37820.20568085966115413No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG37360.20317918870810994TruSeq Adapter, Index 18 (96% over 29bp)
GTCCAGGGTGGGCATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC34960.19012699243135767No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG32820.17848878408458693No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC31820.1730503689692735No Hit
ACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG31050.16886278933048215TruSeq Adapter, Index 18 (96% over 29bp)
CTGCTTCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTG30180.16413136818015947Illumina PCR Primer Index 7 (96% over 27bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT29600.16097708741327768No Hit
ATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG28650.1558105930537299Illumina PCR Primer Index 7 (96% over 27bp)
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGT27600.1501002571826508No Hit
TCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC27060.14716351302038155No Hit
CCCGATGAGCAGCCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGT26850.14602144584616575No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG22420.12192926688532721No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC22150.12046089480419259No Hit
CATCCATAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC21730.11817676045576095No Hit
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGT20990.11415233327042901No Hit
GCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC20890.11360849175889765No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATG20650.11230327213122243No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCAGCTCGTATGC20150.1095840645735657No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC19730.10729993022513408No Hit
CCCGATGAGCAGCACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC18870.10262289322596453No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT18640.10137205774944243No Hit
CATCCGCAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC18400.1000668381217672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)