FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962061
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGC435122.2176680541532603No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGC222541.1342155009451798No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG174520.8894728553291666Illumina PCR Primer Index 10 (96% over 27bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC165950.8457942948766629No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC153910.7844302496201698No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC129500.6600202542122798No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC121140.6174119968747149No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG74820.38133370980820674Illumina PCR Primer Index 10 (96% over 27bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCA70200.35778704127955246No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC59700.30427188553261086No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG58060.29591332787308855No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCA57350.2922946840082954No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATC52600.2680854468846789No Hit
ACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG49650.2530502364605382Illumina PCR Primer Index 10 (96% over 27bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCA44100.22476365413715477No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATC42670.21747539959257128No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCT40870.20830137289309558No Hit
ATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG39880.20325565820838395Illumina PCR Primer Index 10 (96% over 27bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATG35500.18093219323965973No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG31590.1610041685757986No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCA30710.15651908885605492No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATC28160.1435225510317977No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATG26730.13623429648721422No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCA26340.1342465907023278No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATC25920.13210598447245014No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA23280.1186507453132191No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC22750.11594950411837347No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCAT21760.11090378943366185No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCAT21610.11013928720870554No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTC20740.10570517430395895No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGC20660.10529743978398225Illumina PCR Primer Index 10 (95% over 24bp)
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20320.10356456807408129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)