FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002296189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226176
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGC338942.7642035074899525No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGC155381.2671916592724046No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC150401.2265775875567617No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC136401.1124014823320632No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC112080.9140612766845869No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC107220.8744258572994416No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG86330.7040587974320163TruSeq Adapter, Index 4 (96% over 28bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAA52630.4292206012839918No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC51090.416661229709275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG32260.2630943681820554RNA PCR Primer, Index 4 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAAC31600.25771178036431963No Hit
ACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG29850.24343976721123234RNA PCR Primer, Index 4 (96% over 28bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAT27220.22199097030116394No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG27110.22109387233154132No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAA26260.21416175165718462No Hit
ATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG22140.18056135497677334TruSeq Adapter, Index 4 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAT20730.16906219009342868No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAA20200.16473980896706508No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGC18030.1470425126572368TruSeq Adapter, Index 4 (96% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA17690.1442696643874941No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATG17480.14255702280912366No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTT14910.12159755206430398TruSeq Adapter, Index 4 (95% over 21bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG13490.11001683282008455No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAAT12890.10512357116759746No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAA12420.10129051620648259No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAT12360.10080119004123389No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC12310.10039341823685996No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATG12300.10031186387598517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)