FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377591
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG156981.139525446957769TruSeq Adapter, Index 11 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG63820.46327248072904076RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG33890.2460091565638858TruSeq Adapter, Index 11 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC31350.22757117315661907No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG27120.19686539763979294RNA PCR Primer, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG20790.15091562009333684TruSeq Adapter, Index 11 (96% over 28bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC14350.10416734720247156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)