FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002296208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754836
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG383011.3903186977373607RNA PCR Primer, Index 27 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG163140.5921949618779484RNA PCR Primer, Index 27 (96% over 30bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC55190.20033860454851032No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG54800.1989229122895156RNA PCR Primer, Index 27 (96% over 30bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC53280.19340534245958743RNA PCR Primer, Index 27 (95% over 21bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT51580.1872343762024309RNA PCR Primer, Index 27 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG51350.18639948076763915RNA PCR Primer, Index 27 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG50300.1825880016088072RNA PCR Primer, Index 27 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGC49510.17972031728930507No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC41280.14984558064436504RNA PCR Primer, Index 27 (95% over 21bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC36930.1340551669863469RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)