FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002296211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555068
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG170593.073317143124806TruSeq Adapter, Index 10 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG66361.1955291964227808RNA PCR Primer, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG34950.6296525831069347TruSeq Adapter, Index 10 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT30630.5518242809889959No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGC29780.5365108419148645No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG28130.5067847543003741TruSeq Adapter, Index 10 (96% over 28bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT28000.5044426989125657TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG27930.5031815921652842RNA PCR Primer, Index 10 (96% over 28bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC20690.3727471228750351No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC18320.33004965157422156No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC18210.32806791239992217No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC15560.2803260141099829No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG14320.2579864088724264No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13920.25078008460224693No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT12410.22357621048231927No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGA12340.22231510373503788No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG11980.21582941189187632TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGG11080.1996151822839724TruSeq Adapter, Index 10 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10740.19348980665431983No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10300.18556284995712238No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT10200.1837612688895775No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT10170.18322079456931403No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGG8910.16052087311824859RNA PCR Primer, Index 10 (96% over 28bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATGTCGTATGCCGTCTT8880.1599803987979851No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG8720.1570978690899133TruSeq Adapter, Index 10 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG8580.15457565559535047No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG8430.15187328399403316No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG8390.1511526515670152No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG7830.1410637975887639No Hit
CCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA7820.1408836394820094TruSeq Adapter, Index 10 (96% over 28bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG7620.13728047734691964No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG7570.13637968681314722No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT7510.13529873817262028No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG7320.13187573414428502TruSeq Adapter, Index 10 (96% over 28bp)
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAA7190.1295336787564767TruSeq Adapter, Index 10 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT7150.12881304632945872No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7020.12647099094165037No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG6960.12539004230112347No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG6910.12448925176735103No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTT6440.1160218207498901No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC6330.11404008157559074No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC6210.11187818429453687No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG6140.11061707754725547TruSeq Adapter, Index 10 (96% over 28bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG5970.10755438973242919No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT5840.10521233434462084No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT5650.10178933031628556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)