FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6118
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG287446.9761359921543TruSeq Adapter, Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG102816.802876757110166TruSeq Adapter, Index 8 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG64310.509970578620464RNA PCR Primer, Index 8 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG1682.745995423340961TruSeq Adapter, Index 8 (96% over 29bp)
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG1242.0268061457992808TruSeq Adapter, Index 8 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG971.5854854527623405RNA PCR Primer, Index 8 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG370.6047728015691403TruSeq Adapter, Index 8 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG250.40863027133050017RNA PCR Primer, Index 8 (96% over 29bp)
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG210.34324942791762014Illumina PCR Primer Index 2 (96% over 28bp)
CAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG160.2615233736515201TruSeq Adapter, Index 8 (96% over 29bp)
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTG160.2615233736515201TruSeq Adapter, Index 8 (96% over 29bp)
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA130.21248774109186006TruSeq Adapter, Index 8 (96% over 29bp)
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAA120.1961425302386401TruSeq Adapter, Index 8 (96% over 29bp)
TACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGG110.17979731938542007TruSeq Adapter, Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG90.14710689767898005TruSeq Adapter, Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG90.14710689767898005Illumina PCR Primer Index 8 (96% over 27bp)
GTACTCGTCAGTGTAAATCTCTTGAATTTACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA90.14710689767898005No Hit
CATATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG90.14710689767898005TruSeq Adapter, Index 8 (96% over 29bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTG80.13076168682576006TruSeq Adapter, Index 8 (96% over 29bp)
GTGTAAATCTCTTGAATTTACACTGACGAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA80.13076168682576006No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG80.13076168682576006Illumina PCR Primer Index 8 (96% over 27bp)
ACAGCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG80.13076168682576006TruSeq Adapter, Index 8 (96% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.11441647597254005No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTATTCTGCTTGAAAAAAAAAAGGGGG70.11441647597254005Illumina PCR Primer Index 8 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG70.11441647597254005TruSeq Adapter, Index 8 (96% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG70.11441647597254005TruSeq Adapter, Index 8 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)