FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG915.620753551575047No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG794.879555281037677No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG684.2001235330450895No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC633.891290920321186No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG613.7677578752316245No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT603.7059913526868438No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA573.5206917850525015No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT392.4088943792464486No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT382.347127856701668No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT372.285361334156887No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT352.1618282890673255No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA342.1000617665225447No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA301.8529956763434219No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC301.8529956763434219No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGCCAATA301.8529956763434219No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT271.6676961087090798No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA271.6676961087090798No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT231.4206300185299567No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC211.2970969734403952No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCGCTTGGCCTTGTCGCGGCTCTTG211.2970969734403952No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA150.9264978381717109No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT130.8029647930821495No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCACTGCTGCAGAACTTGATAGCCACACC110.6794317479925881No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGTCCTGCTTGAGGATGGGTAGGGGGAT100.6176652254478072No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC90.5558987029030266No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC90.5558987029030266No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCTTGGAAGGCGGGGACCCAACCTTCCCAC90.5558987029030266No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGNGCCATTCAGGTA80.49413218035824585No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGNGAAGATGCCGAT80.49413218035824585No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCNCCTGCGCACATG70.4323656578134651No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGTCCTGCTTGGGGATGGGTAGGGGGAT70.4323656578134651No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC70.4323656578134651No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCACTGCTGCAGAACTTGATAGCCACAC70.4323656578134651No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGNGAAGGGGCCCTG60.3705991352686844No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCNATTGGGATCATG50.3088326127239036No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGNTATCAGCCAAGC50.3088326127239036No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCAC50.3088326127239036No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGTTGACAACAGTCA50.3088326127239036No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG50.3088326127239036No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCACTG40.24706609017912293No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT40.24706609017912293No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT40.24706609017912293No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTTCCCCTGCGCACATG40.24706609017912293No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTTTAATATGTGCTTTAGACTCTCCACT40.24706609017912293No Hit
AGCCAAAGGGTCTAAAGAAGTGGAAGTCACTCTTGTTCGGAAAGACAAAGGGCGCCAGAAAACAACCCGATCACG40.24706609017912293No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCNTGGCAGTATTAT40.24706609017912293No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACATGTGCACCACTTCTGCCCCTGCGCACATG40.24706609017912293No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTTTAATATGTGCTTTAGACTCTCCACT40.24706609017912293No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACGTCTGCCCCTGCGCACATG40.24706609017912293No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCACGT30.1852995676343422No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTACTGGAAGGCGGGGACCCAACCTTCCCAC30.1852995676343422No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACNCTTAACGGCCCC30.1852995676343422No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCGCTTGGCCTTNTCGCGGCTCTTG30.1852995676343422No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCNGGTAGCCGGCAA30.1852995676343422No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCATGTA30.1852995676343422No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAA30.1852995676343422No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGTTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG30.1852995676343422No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGACTGGCAGGTCGCGAAGGGGCCCTG30.1852995676343422No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAACCGGAGTGGTGCCATTCAGGTA30.1852995676343422No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA30.1852995676343422No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGTGTGCGGGTGGCCCCCTCAGTGA30.1852995676343422No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGTGGCCCCCTCAGTGA20.12353304508956146No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGACGGCAA20.12353304508956146No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCGAATTTCAGCTCCATTGGGATCATG20.12353304508956146No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGG20.12353304508956146No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAG20.12353304508956146No Hit
CCTTTAACCAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT20.12353304508956146No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTTCCAATTTCAGCTCCATTGGGATCATG20.12353304508956146No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACGCATCCTGCAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC20.12353304508956146No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGNTATCAGCCAAGT20.12353304508956146No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCACTTTCAGCTCCATTGGGATCATG20.12353304508956146No Hit
GCACAAGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG20.12353304508956146No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATACGCATCACTCTGCACAGCATCATCCAT20.12353304508956146No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTTCGCTGCTTGGCCTTGTCGCGGCTCTT20.12353304508956146No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCATCACTCTGCACAGCATCATCCAT20.12353304508956146No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACACTCAGCACCCTTTCAACAACTCCTGTT20.12353304508956146No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCACTGCTGCAGACCTTGATAGCCACAC20.12353304508956146No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCCAGC20.12353304508956146No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGAGTTGCCAATTTCAGCTCCATTGGGATCATG20.12353304508956146No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCATCGGGTAGCCGGCAA20.12353304508956146No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGCTGCGGGGGGCCCCCTCAGTGA20.12353304508956146No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTTCGCTGTATTCTCCTAAGATGGGCTC20.12353304508956146No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGNCCCCCTCAGTGA20.12353304508956146No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGTCCGGGGCACCAGCAGCGGGTAGCCGGCAA20.12353304508956146No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCGGGTCGCGAAGGGGCCCTG20.12353304508956146No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCATG20.12353304508956146No Hit
CTTTGACAGCATCACGAGTTTCACCGGTATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG20.12353304508956146No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACCTG20.12353304508956146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)