FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002296283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT444.44444444444444No Hit
GTCATTGTCACTGTTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT111.11111111111111No Hit
GCACAGGTCGTATACGCATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAACA111.11111111111111Illumina PCR Primer Index 2 (96% over 28bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGTCCTTGTCGCGGCTCTT111.11111111111111No Hit
GTCATTATCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT111.11111111111111No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGACAAGCGTGTATCTCGTATGCCGTCTT111.11111111111111TruSeq Adapter, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)