FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002296285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT838.095238095238095No Hit
GCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTTG14.761904761904762No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTTCTTGGCCTTGTCGCGGCTCTT14.761904761904762No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTNGTCGCGGCTCTT14.761904761904762No Hit
GTCATTGTCATGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTTG14.761904761904762No Hit
GTCATTGTCACTTGTCAGCTCCAGCACCTTCGGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCGCTT14.761904761904762No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTTCGCTGCTTGGCCTTATCGCGGCTCTT14.761904761904762No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCTTNTTCGCGGTTCTT14.761904761904762No Hit
CAAGCAATCAAGGCCAGTCTTTATAACATCTCCGAGCCCACGAGACGATGCACAATCTCGTCTGCCGTCTTCTTC14.761904761904762RNA PCR Primer, Index 36 (95% over 22bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCTCGTCTCTT14.761904761904762No Hit
GACATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT14.761904761904762No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGACTTGTCGCGGCGCTT14.761904761904762No Hit
CAACCAAAAGAACAGCCTGTCTCTTTATACATCTCCGAGCCCACGCGACGATGCACAATCTCGTATGCCGTCTTC14.761904761904762Illumina PCR Primer Index 1 (95% over 22bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCGCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)