FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002296286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG838.095238095238095No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT14.761904761904762RNA PCR Primer, Index 45 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACGTGCATTCATCTCGTATGCCGTCTTC14.761904761904762RNA PCR Primer, Index 45 (95% over 24bp)
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCAGAGACGTGCATTCCTCTCGTCTTCCGTCTTC14.761904761904762No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGTGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG14.761904761904762No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCCTTCATCTCGTATGCCTTCTT14.761904761904762No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGTGCATTCATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA14.761904761904762TruSeq Adapter, Index 7 (96% over 26bp)
CAACCAAAAGAACAGCCTGTCGCTTATACAAATGTCCGAGCCCAAAAGACGTGCAGGAATATCGTATGCCGTCGT14.761904761904762No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGAGACGTGCATTCAACTCGAATGCAGTCTTC14.761904761904762No Hit
CAACCAAAAGAACAGCCTATCTCTTATACACATCTCCGAGCCCACGAGCCGTGCATTCAACTCGTATGCCGTCTT14.761904761904762No Hit
GCACAGGTCGTATACGCAGTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGAGAAGGGGCCCTG14.761904761904762No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGCGACTCAGCACCGCCTGGCAGGTCGCGAACGGGCCCTG14.761904761904762No Hit
GCCCAGGTCGTATACGCATTGGCGGAAGTACTCAGAGTGACTCAGCACCGCCTGTCAGGTCACGAAGTGGCCCTG14.761904761904762No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCGG14.761904761904762No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)