FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC25813.694267515923567No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC1507.961783439490445No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG1186.263269639065817No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG1065.626326963906582No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT995.254777070063694No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG995.254777070063694No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT965.095541401273886No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC713.768577494692144No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT603.1847133757961785No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT562.9723991507431No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG542.8662420382165608No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC522.7600849256900215No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA452.388535031847134No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC412.176220806794055No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG392.0700636942675157No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG341.8046709129511678No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG301.5923566878980893No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT291.5392781316348196No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG251.3269639065817411No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA251.3269639065817411No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG251.3269639065817411No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT241.2738853503184715No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG241.2738853503184715No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT201.0615711252653928No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT120.6369426751592357No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG110.583864118895966No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT80.42462845010615713No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC80.42462845010615713No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAC40.21231422505307856No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC40.21231422505307856No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACACTCGCTAATCTCGT40.21231422505307856No Hit
TTTCAGGTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT40.21231422505307856Illumina PCR Primer Index 10 (95% over 23bp)
CTCTCAGCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC40.21231422505307856Illumina PCR Primer Index 10 (95% over 22bp)
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT40.21231422505307856No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA40.21231422505307856No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC40.21231422505307856No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT40.21231422505307856No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC30.15923566878980894No Hit
GGCTGTGGTGGGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC30.15923566878980894No Hit
CATAGTTACCTTCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG30.15923566878980894No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT30.15923566878980894No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCACCATTTGCACCCGC30.15923566878980894No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT30.15923566878980894No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGTTGCGTCTCCACGTTGC20.10615711252653928No Hit
TAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT20.10615711252653928No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCCCCGGGGCGGCAGGTGGGGCG20.10615711252653928No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT20.10615711252653928No Hit
GTAAAAGTTGAAATGTCAGGGCCCACGAGACACTCGCTAATCTCGTATGC20.10615711252653928No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCCCCACGTTGC20.10615711252653928No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGTAGGTGGGGCG20.10615711252653928No Hit
AGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC20.10615711252653928No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG20.10615711252653928No Hit
TTTCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCG20.10615711252653928No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT20.10615711252653928No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA20.10615711252653928No Hit
ATCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC20.10615711252653928No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA20.10615711252653928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)