FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT2116.956808440487966No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG1876.165512693702604No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT1685.539070227497527No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA1605.275304978569074No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG1444.747774480712167No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC1354.451038575667656No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC1354.451038575667656No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG1254.121332014507089No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG1113.6597428288822944No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG1023.3630069238377844No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC1013.3300362677217277No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT953.132212331025387No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG913.0003297065611605No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG913.0003297065611605No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG862.835476425980877No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA832.736564457632707No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT822.70359380151665No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG511.6815034619188922No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT401.3188262446422685No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG341.1210023079459281No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT311.022090339597758No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT280.9231783712495879No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC280.9231783712495879No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT250.8242664029014177No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC220.7253544345532476No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT170.5605011539729641No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA110.3626772172766238No Hit
TTTCAGGTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT80.26376524892845365Illumina PCR Primer Index 10 (95% over 23bp)
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT70.23079459281239698No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA60.19782393669634024No Hit
CTCTCAGCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC60.19782393669634024Illumina PCR Primer Index 10 (95% over 22bp)
CCTGACTCACGTTTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCG60.19782393669634024No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGTGGGGACCG50.16485328058028356No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACACTCGCTAATCTCGT40.13188262446422683No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC40.13188262446422683No Hit
CTGGAATCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT40.13188262446422683Illumina PCR Primer Index 10 (95% over 23bp)
TTTCAGGTCAGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCT40.13188262446422683RNA PCR Primer, Index 29 (95% over 22bp)
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT40.13188262446422683No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)