FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154496
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT84675.480400787075394No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA54573.5321302816901405No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG53933.490705260977631No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG36302.349575393537697No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC31702.0518330571665286No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC29611.916554473902237No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT27341.7696251035625519Illumina PCR Primer Index 10 (95% over 21bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA26661.7256110190555094No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG25951.6796551367025685No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGAC24591.591626967688484No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT23651.5307839685169844No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT22491.4557011184755593No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT21231.3741456089478044Illumina PCR Primer Index 10 (95% over 23bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT15050.9741352526926264No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT14450.9352992957746479No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACAT13850.8964633388566694No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGA13820.8945215410107706No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT12980.8401512013256007No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT12410.8032570422535211No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT12250.7929007870753936No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC11290.7307632560066281No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACC10140.656327671913836No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT10110.654385874067937No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC9880.6394987572493787No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT8660.5605323115161558No Hit
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCT8330.5391725352112676No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCAC8280.5359362054681027No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTC7410.479624067937034No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA7110.46020608947804476No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT6940.4492025683512842No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC6650.43043185584092797No Hit
GTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATC6650.43043185584092797No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTC6560.4246064623032311No Hit
GTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTG6540.4233119304059652No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT6350.41101387738193873No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACA6240.40389395194697597No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG5820.37670878210439107Illumina PCR Primer Index 10 (95% over 24bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC5800.3754142502071251No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC5710.36958885666942837No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG5570.36052713338856673No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACAT5480.3547017398508699No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTG5480.3547017398508699No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA5320.34434548467274234No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC5210.33722555923777964No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTG5190.33593102734051367No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCT5190.33593102734051367No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG4980.32233844241922116No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCT4830.31262945318972657No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTC4550.29450600662800336No Hit
AAAGTTGAAATGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCG4510.2919169428334714No Hit
AAAGTTGAAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTC4250.27508802816901406No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTG3910.253080985915493No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTA3780.2446665285832643No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATA3770.24401926263463133No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTC3750.2427247307373654No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGA3720.24078293289146643No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCT3700.2394884009942005No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCC3600.23301574150787077No Hit
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCT3460.22395401822700914No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTA3450.22330675227837612No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACAT3450.22330675227837612No Hit
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAAT3410.22071768848384424No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA3350.2168340927920464No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACA3250.21036143330571666No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCT3230.2090669014084507No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTC3210.20777236951118475No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA3200.2071251035625518No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTG3200.2071251035625518No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC3100.20065244407622204No Hit
GATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTG3030.19612158243579123No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCCTGTCTCTTATACAC2700.17476180613090306No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT2690.1741145401822701No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG2660.17217274233637117No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGC2650.17152547638773818No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACA2650.17152547638773818No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT2630.17023094449047224Illumina PCR Primer Index 10 (96% over 26bp)
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTA2600.16828914664457334No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTCTGTCTCTTATACACATCTC2550.16505281690140844No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT2490.16116922120961058No Hit
GTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCT2470.15987468931234466No Hit
CATCCGCAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAC2440.15793289146644574No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCG2420.1566383595691798No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCT2320.15016570008285005No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCT2320.15016570008285005No Hit
AAAGTTGAAATGTCAGGGTCACGTCCCTGTCTCTTATACACATCTCCGAG2310.1495184341342171No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCT2250.14563483844241923No Hit
TTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTA2200.14239850869925436No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC2200.14239850869925436No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCT2170.14045671085335543No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCT2160.13980944490472244No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCT2160.13980944490472244No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA2160.13980944490472244No Hit
AAAGTTGAAATGTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA2110.13657311516155757No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2060.13333678541839272No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGT2030.1313949875724938No Hit
AAAGTTGAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC1980.1281586578293289No Hit
GTTTCCAGGTCAAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1980.1281586578293289No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAG1960.12686412593206298No Hit
CATCCATAGCGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAC1960.12686412593206298No Hit
GTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA1910.1236277961888981No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTTTGCCGT1890.12233326429163215No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC1890.12233326429163215No Hit
ATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT1890.12233326429163215No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTC1880.12168599834299917No Hit
CTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCT1860.12039146644573322No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATATCGTATGCCGT1820.11780240265120133No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT1810.11715513670256836No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGC1800.11650787075393539No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAC1790.1158606048053024No Hit
GCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGA1750.1132715410107705No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTC1730.11197700911350457No Hit
CATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC1710.11068247721623861No Hit
GCTCTCAGCTCCGTAGTGTGCTGTCTCTTATACACATCTCCGAGCCCACG1700.11003521126760564No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTGT1690.10938794531897265Illumina PCR Primer Index 10 (95% over 21bp)
CATCCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAA1690.10938794531897265No Hit
CCCGATGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACG1670.10809341342170671No Hit
ATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCC1640.10615161557580778No Hit
GACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCC1610.10420981772990887No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCT1600.1035625517812759No Hit
GATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGAT1600.1035625517812759No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATCTGTC1590.10291528583264292No Hit
GCGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTT1580.10226801988400994No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTC1570.10162075393537696No Hit
CATCCATAGCGTCCTCAGCTGTCTCTGTCTCTTATACACATCTCCGAGCC1560.10097348798674399No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)