FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730213
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT261541.5116057965117589No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG111010.6415973062276147No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC96210.5560587049108983No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA93420.5399335226356524No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT82510.4768777023406945RNA PCR Primer, Index 47 (95% over 22bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG65940.3811091466773166No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT60870.3518063960911171RNA PCR Primer, Index 47 (95% over 24bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG56170.32464211053783554No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG43250.24996922344243167No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTC40310.2329770958835704No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTT37690.21783445159642192No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT36900.21326853976938098No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC34630.20014876781066837No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATAC31920.18448595635335072No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCA27460.1587087832538537No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCT26590.15368050060888455No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACAC26500.15316033343871535No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATAC25480.14726510551013083No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACA25180.14553121494290008No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCT22490.12998399619006445No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTT22470.12986840348558243No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACAT21890.12651621505560298No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21430.12385758285251584Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCT20270.11715320599255698No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19960.11536151907308523Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTT19320.11166255252965963No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19110.11044882913259811Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACAC18800.10865714221312636No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACAT18450.10663426988469049No Hit
GTATCAACGCAGAGTACATGGGGTCTCGTGGTCACAGGTAAGTGCTAAAA18170.10501597202194181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)