FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002296389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913524
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGC144291.5794877857615126No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG104161.140199929065903Illumina PCR Primer Index 6 (96% over 27bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGC73080.7999789824897867No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC70850.7755680201067514No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC66340.7261987643455454No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC56340.616732565318481No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC49210.538683165412184No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG40210.44016358628782604Illumina PCR Primer Index 6 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG32880.3599248624009878Illumina PCR Primer Index 6 (96% over 27bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATA31550.34536585793038826No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC23990.26260941146592753No Hit
ATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG23160.25352371694668124Illumina PCR Primer Index 6 (96% over 27bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC18640.20404499498644807No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG18280.20010421182147375No Hit
CATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG16340.17886776921022327Illumina PCR Primer Index 6 (96% over 27bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT15890.17394179025400536No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTA15590.17065780428319344No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC14610.15993011677854113No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT13760.15062548986124066No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATG13560.14843616588069936No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT11950.13081210783734198No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG11190.1224926767112851No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10760.11778563015312131No Hit
CACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG10660.11669096816285067RNA PCR Primer, Index 37 (96% over 28bp)
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATG10310.11285965119690342No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT9910.10848100323582084No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG9900.10837153703679378Illumina PCR Primer Index 6 (96% over 27bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC9850.10782420604165846No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9850.10782420604165846No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT9780.10705794264846902No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC9650.10563488206111717No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT9240.10114676790100752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)