FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826066
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG205911.1276153216805964RNA PCR Primer, Index 39 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC73110.40036888042381824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG65380.3580374422392181RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG40850.22370494823297735RNA PCR Primer, Index 39 (96% over 30bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC33980.18608308790591357RNA PCR Primer, Index 39 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC30180.16527332527959013RNA PCR Primer, Index 39 (95% over 21bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC29710.16269948621791328RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG26390.1445183251864938RNA PCR Primer, Index 39 (96% over 30bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC18950.10377500046548155RNA PCR Primer, Index 39 (95% over 21bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG18380.100653536071533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)