FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16245
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA1921.1819021237303784No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1731.0649430594028932TruSeq Adapter, Index 3 (96% over 28bp)
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA1340.8248691905201601No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAA1320.8125577100646353No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA1170.7202216066481995No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATG970.5971068020929517No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAA840.5170821791320407No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG810.4986149584487534RNA PCR Primer, Index 3 (96% over 28bp)
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA800.49245921822099104No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCG760.46783625730994155No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC660.40627885503231764No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATG640.3939673745767929No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA600.3693444136657433No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG570.3508771929824561No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC570.3508771929824561No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGT540.33240997229916897No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCG540.33240997229916897No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA530.3262542320714066No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT530.3262542320714066No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT520.3200984918436442No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA520.3200984918436442No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGT520.3200984918436442No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC500.3077870113881194No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC440.2708525700215451No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA430.26469682979378273No Hit
TTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCG410.2523853493382579No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCG410.2523853493382579No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG400.24622960911049552No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA390.24007386888273313No Hit
AAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT390.24007386888273313No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC380.23391812865497078No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC370.2277623884272084No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA360.221606648199446No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGA340.2092951677439212No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGC340.2092951677439212No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG330.20313942751615882No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG330.20313942751615882No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC320.19698368728839644No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG320.19698368728839644No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA320.19698368728839644No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC320.19698368728839644No Hit
AAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCT320.19698368728839644No Hit
TCTCTTGAATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC310.19082794706063405No Hit
GAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC310.19082794706063405No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA310.19082794706063405No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.17851646660510925No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGA290.17851646660510925No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.17851646660510925No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA280.1723607263773469No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG280.1723607263773469No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG280.1723607263773469No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACG280.1723607263773469No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC280.1723607263773469No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG270.16620498614958448No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCG270.16620498614958448No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA270.16620498614958448No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA270.16620498614958448No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC270.16620498614958448No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA270.16620498614958448No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG260.1600492459218221No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTCG260.1600492459218221No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG260.1600492459218221No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA260.1600492459218221No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA250.1538935056940597No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTC250.1538935056940597No Hit
GTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCG250.1538935056940597No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG240.1477377654662973TruSeq Adapter, Index 3 (96% over 28bp)
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA240.1477377654662973No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAG240.1477377654662973No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC230.14158202523853494No Hit
GTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC230.14158202523853494No Hit
GTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCG230.14158202523853494No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT230.14158202523853494No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGA230.14158202523853494No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA220.13542628501077256No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC220.13542628501077256No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAG220.13542628501077256No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA220.13542628501077256No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAA220.13542628501077256No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA220.13542628501077256No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAG210.12927054478301017No Hit
AGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAA210.12927054478301017TruSeq Adapter, Index 3 (96% over 28bp)
TTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAA210.12927054478301017No Hit
GGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG200.12311480455524776No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA200.12311480455524776No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAG200.12311480455524776No Hit
GTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCT200.12311480455524776No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA200.12311480455524776No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA190.11695906432748539No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG190.11695906432748539No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG190.11695906432748539No Hit
TGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCT190.11695906432748539No Hit
TTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTCG190.11695906432748539No Hit
CATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCG180.110803324099723No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCA180.110803324099723No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCAC180.110803324099723No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTC180.110803324099723No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGA180.110803324099723No Hit
CTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG170.1046475838719606No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG170.1046475838719606No Hit
TCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC170.1046475838719606No Hit
CCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA170.1046475838719606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)