FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30997
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG17635.687647191663709TruSeq Adapter, Index 1 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC7252.3389360260670387No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG6762.180856211891473RNA PCR Primer, Index 1 (96% over 29bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA3731.2033422589282834No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG3231.0420363260960737TruSeq Adapter, Index 1 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG2500.8065296641610478No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT1930.6226409007323289No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA1880.6065103074491079No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT1860.6000580701358196No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA1830.590379714165887No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT1370.4419782559602542No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1250.4032648320805239TruSeq Adapter, Index 1 (96% over 29bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG1190.3839081201406588No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG1180.3806820014840146TruSeq Adapter, Index 1 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC1150.371003645514082No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG1120.36132528954414944No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA1040.33551634029099586No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1010.3258379843210633No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG990.31938574700777494No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC980.31615962835113076No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG950.30648127238119816TruSeq Adapter, Index 1 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG880.2838984417846888RNA PCR Primer, Index 1 (96% over 29bp)
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA860.27744620447140045No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG810.26131561118817953RNA PCR Primer, Index 1 (96% over 29bp)
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC790.2548633738748911No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGA750.24195889924831437TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG720.23228054327838177TruSeq Adapter, Index 1 (96% over 29bp)
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT700.22582830596509337TruSeq Adapter, Index 1 (95% over 21bp)
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG660.2129238313385166No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA650.20969771268187243No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCG640.20647159402522827No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG640.20647159402522827No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC630.20324547536858406No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA630.20324547536858406No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC600.19356711939865148No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT580.1871148820853631TruSeq Adapter, Index 1 (95% over 24bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG560.18066264477207472No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA560.18066264477207472No Hit
GTACTACCGACCCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA550.1774365261154305No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT550.1774365261154305No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATG550.1774365261154305No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT540.17421040745878635No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG540.17421040745878635TruSeq Adapter, Index 1 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG540.17421040745878635RNA PCR Primer, Index 1 (96% over 29bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA530.17098428880214214No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC520.16775817014549793No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA510.16453205148885378No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGC510.16453205148885378No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACG510.16453205148885378No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC500.16130593283220956No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC490.15807981417556538No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC490.15807981417556538No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG480.1548536955189212TruSeq Adapter, Index 1 (96% over 29bp)
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC470.15162757686227699No Hit
GTACTACCGACCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCG460.1484014582056328No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC460.1484014582056328No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT460.1484014582056328TruSeq Adapter, Index 1 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG440.1419492208923444No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC440.1419492208923444No Hit
GTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAA440.1419492208923444TruSeq Adapter, Index 1 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGG430.13872310223570022No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGG430.13872310223570022RNA PCR Primer, Index 1 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC420.13549698357905604No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAG400.12904474626576767No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT400.12904474626576767TruSeq Adapter, Index 1 (95% over 22bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC390.12581862760912346No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG390.12581862760912346No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC390.12581862760912346No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC390.12581862760912346No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACG380.12259250895247926No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG380.12259250895247926TruSeq Adapter, Index 1 (96% over 26bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCT380.12259250895247926No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC370.11936639029583508No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTGTGCTTGAAAAAAAAAAGGGGG370.11936639029583508Illumina Paired End PCR Primer 2 (96% over 25bp)
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT360.11614027163919088No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCG360.11614027163919088No Hit
GATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGC350.11291415298254669No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC350.11291415298254669No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT340.10968803432590252TruSeq Adapter, Index 1 (95% over 22bp)
CGTCAGATGTGTATAAGAGACAGGTACTACCGACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC340.10968803432590252No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCG340.10968803432590252No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG330.1064619156692583Illumina Paired End PCR Primer 2 (96% over 25bp)
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT320.10323579701261414No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)