FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227997
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG16540.7254481418615157Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG7030.3083373904042597Illumina PCR Primer Index 11 (96% over 29bp)
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA4360.19123058636736448No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG4090.1793883252849818No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG3170.13903691715241867Illumina PCR Primer Index 11 (96% over 29bp)
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA2920.12807186059465694No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA2580.11315938367610101No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT2410.10570314521682303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)