FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002296478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC120.0No Hit
CAACCAAAAGAACAGCCTTTCTCTTATACACATCCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTC120.0RNA PCR Primer, Index 37 (95% over 23bp)
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA120.0RNA PCR Primer, Index 47 (96% over 30bp)
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC120.0No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGTTGGAAGAGCTCGTATGCCGTCTTCTACTTTAAAAAAAAAAAAAA120.0Illumina Single End Adapter 1 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)