FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002296483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT1666.66666666666666No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCATTGTCGCGGCTCTT28.333333333333332No Hit
GTCATTGTCACTGGTCATCTCCAGCACCTTCTGCTGCGTCTCCACGTTACGCTGCTGGGCCTTGTCGCGGCTCTT14.166666666666666No Hit
GTCATTGTCACTGGTCAACTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCGTTGTCGCGGCTCTT14.166666666666666No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCAACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT14.166666666666666No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACTTTGCGCTGCTTGTCCTTGTCGCGGCTCTT14.166666666666666No Hit
CCTGACTCACATCTCCGCGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGA14.166666666666666Illumina Paired End PCR Primer 2 (96% over 28bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCGTTGTCGCGGCTCTT14.166666666666666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)