FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270423
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC37830.29777483562561446No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG17050.13420726797295074TruSeq Adapter, Index 7 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG13910.10949109076268297No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC13620.1072083864980404No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)