FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA220.5467196819085487No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG190.4721669980119284No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.4224652087475149No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA170.4224652087475149No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA170.4224652087475149No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG150.3727634194831014No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG150.3727634194831014No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATC140.34791252485089463No Hit
CTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG140.34791252485089463TruSeq Adapter, Index 1 (96% over 28bp)
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.34791252485089463No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC130.3230616302186879No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC130.3230616302186879No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA130.3230616302186879No Hit
TTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG130.3230616302186879No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA120.2982107355864811No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCAC110.27335984095427435No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG110.27335984095427435No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA110.27335984095427435No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG100.2485089463220676RNA PCR Primer, Index 1 (96% over 28bp)
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT100.2485089463220676No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC100.2485089463220676No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA100.2485089463220676No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC100.2485089463220676No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA100.2485089463220676No Hit
GCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTGATGAACCCTGGAATACTGGAGCGACGCGTGAT90.22365805168986083No Hit
AGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGGTTCATC90.22365805168986083No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA90.22365805168986083No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT90.22365805168986083No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGA90.22365805168986083No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATC80.19880715705765406No Hit
TCTCTTGAATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC80.19880715705765406No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT80.19880715705765406No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA80.19880715705765406No Hit
GGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCAC80.19880715705765406No Hit
CTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG80.19880715705765406No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCC80.19880715705765406No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA80.19880715705765406No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC80.19880715705765406No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGA80.19880715705765406No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCAC70.17395626242544732No Hit
GAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC70.17395626242544732No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG70.17395626242544732No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT70.17395626242544732No Hit
CAAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATT70.17395626242544732No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC70.17395626242544732No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGA70.17395626242544732No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA70.17395626242544732No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC70.17395626242544732No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC70.17395626242544732No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC70.17395626242544732No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60.14910536779324055No Hit
AGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC60.14910536779324055No Hit
ATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCAC60.14910536779324055No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCAC60.14910536779324055No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60.14910536779324055No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC60.14910536779324055No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC60.14910536779324055No Hit
GAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCA60.14910536779324055No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG60.14910536779324055No Hit
GAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCT60.14910536779324055No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA60.14910536779324055No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT60.14910536779324055No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCT50.1242544731610338No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATG50.1242544731610338No Hit
GTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC50.1242544731610338No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAG50.1242544731610338No Hit
ACCTGGAGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTGATGAACCCTGGAATACTGG50.1242544731610338No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCA50.1242544731610338No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA50.1242544731610338No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.1242544731610338No Hit
ATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCGTGCTTTCTTGG50.1242544731610338No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCAC50.1242544731610338No Hit
CTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCGTGCTTT50.1242544731610338No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG50.1242544731610338No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA50.1242544731610338No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATC50.1242544731610338No Hit
TTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA50.1242544731610338No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA50.1242544731610338No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCT50.1242544731610338No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT50.1242544731610338No Hit
GATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGT50.1242544731610338No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG50.1242544731610338No Hit
CAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT50.1242544731610338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)