FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16993
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC515730.347790266580354No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT400923.592067321838403No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC334919.70811510622021No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT9855.796504443005944No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG9655.6788089213205435No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2131.2534573059495087No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT1701.000411934325899No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC720.42370387806743953No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCT600.3530865650561996No Hit
GGCTGTGGTGTGAGTCCTCTGGTGGGCCTTTAAGTGGGAGCTTTTTGTAT520.30600835638203966No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG470.2765844759606897No Hit
GTTTCCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTT380.22362149120225974No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG350.20596716294944978No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG300.1765432825280998No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.13534984993820984No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG210.12358029776966986No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT200.11769552168539987No Hit
TTTCAGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCC190.11181074560112987No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGCTTTGC180.10592596951685988No Hit
TTTCAGGTCAGAGCTGAAGTCCCCACGACGTTTGCCCTCTGTGGATTTGC180.10592596951685988No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.10004119343258988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)