FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31314
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG353211.27929999361308No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG25748.219965510634221No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC24217.731366162100019No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG21496.862745098039216No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT21466.853164718656192No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT17945.729066871048094No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC16155.157437567861021No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT13684.368652998658747No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG13094.180238870792617No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT12804.087628536756722No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC11543.685252602669732No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC10113.228587852078942No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT9463.021012965446765No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT7872.5132528581465157No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG7212.3024845117199972No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG4971.587149517787571No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG4431.4147026888931469No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA4421.4115092290988056No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC4141.3220923548572523No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT4121.31570543526857No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG3271.044261352749569No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG3020.9644248578910392No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA2370.7568499712588619No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT1750.5588554640097081No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA1380.44069745161908414No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC540.17244682889442423No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT450.14370569074535353No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG330.10538417321325924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)