FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC706.979062811565304No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT565.583250249252243No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG555.483549351944168No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG525.18444666001994No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC504.985044865403789No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT494.885343968095713No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG434.287138584247258No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG434.287138584247258No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA403.988035892323031No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT353.489531405782652No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG292.8913260219341974No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC282.7916251246261217No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT272.6919242273180455No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG262.59222333000997No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG262.59222333000997No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC242.3928215353938187No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG242.3928215353938187No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA201.9940179461615155No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT191.8943170488534395No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG171.694915254237288No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG171.694915254237288No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT171.694915254237288No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT131.296111665004985No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT111.0967098703888334No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC80.7976071784646062No Hit
TTTCAGGTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT70.6979062811565304Illumina PCR Primer Index 10 (95% over 23bp)
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA70.6979062811565304No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT60.5982053838484547No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG60.5982053838484547No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG50.4985044865403789No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT40.3988035892323031No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACACTCGCTAATCTCGT40.3988035892323031No Hit
CTGGAATCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT30.29910269192422734Illumina PCR Primer Index 10 (95% over 23bp)
CTCTCAGCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC30.29910269192422734Illumina PCR Primer Index 10 (95% over 22bp)
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAAACTCGT30.29910269192422734No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC20.19940179461615154No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.19940179461615154No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCG20.19940179461615154No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCC20.19940179461615154No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACACTCGCTAATCTCGT20.19940179461615154No Hit
TGGACAAGAACAGCAACGAGCCCACGAGACACTCGCTAATCTCGTATGCC20.19940179461615154No Hit
GCACAGGTCGCATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG20.19940179461615154No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA20.19940179461615154No Hit
CATAGTTACCTTCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG20.19940179461615154No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACGATGCACAATCTCGT20.19940179461615154No Hit
ATGCTCAGTTGTAGGTGATTCTCTTGCAGGAACTCACCCACTCATCCTGC20.19940179461615154No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCG20.19940179461615154No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCTCGT20.19940179461615154No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAACCTCGT20.19940179461615154No Hit
CCTGACTCACATTTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG20.19940179461615154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)