FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63033
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC792112.566433455491568No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC703611.162406993162312No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT667210.584931702441578No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG62219.869433471356274No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG49027.776878777783066No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG46127.316802309901163No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG36685.819174083416623No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC28264.4833658559802005No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC28184.470674091348976No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT22053.498167626481367No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG20743.290339980645059No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT17912.8413688068154777No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG12912.0481335173639206No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA12762.0243364586803736No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA6891.0930782288642458No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA5970.9471229356051593No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2700.42834705630384085No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT1820.2887376453603668No Hit
GGCTGTGGTGTGAGTCCGGTGGGGCGGCCGTAGCGCGCCATTTGCACCCG1510.23955705741437028No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCC1500.23797058683546712No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG830.1316770580489585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)