FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002296548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717781
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG168992.354339276185912TruSeq Adapter, Index 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG60240.8392531983989546RNA PCR Primer, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG57540.80163726819183TruSeq Adapter, Index 1 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG46190.643511043061881RNA PCR Primer, Index 1 (96% over 28bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC19220.2677696957707156No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG18830.2623362836296865TruSeq Adapter, Index 1 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGC17100.23823422464512156No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC15410.2146894387006622No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG13120.18278555715461958TruSeq Adapter, Index 1 (96% over 28bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC12840.17888464587388075No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC11830.16481350161121566No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG11550.16091259033047686TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG11190.15589713296952692TruSeq Adapter, Index 1 (96% over 28bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT10770.15004576604841866TruSeq Adapter, Index 1 (95% over 21bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT10480.14600553650765347No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATG8810.12273938708324685No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8140.11340506366147893No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGA7860.10950415238074009No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGAT7770.1082502880405026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)