FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33521
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGC10473.1234151725783836No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG5801.7302586438352079No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTA4791.4289549834432147No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG4641.3842069150681662No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTA4571.3633244831598101No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG4561.3603412786014737No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG4321.2887443692013962RNA PCR Primer, Index 41 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA2610.7786163897258435No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG2470.7368515259091316No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1970.5876912979923034No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1890.5638256615256108RNA PCR Primer, Index 41 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT1810.5399600250589183No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACA1730.5160943885922258No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT1640.4892455475671967No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC1450.43256466095880197No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT1430.42659825184212885No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA1400.4176486381671191No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTA1370.40869902449210943No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC1370.40869902449210943No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAA1340.3997494108170997No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT1320.39378300170042657No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACC1280.3818501834670804No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC1210.3609677515587244No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGC1190.3550013424420512No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT1110.33113570597535874RNA PCR Primer, Index 41 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG1010.3013036603919931RNA PCR Primer, Index 41 (96% over 29bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAA960.2863876376003102No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGC950.28340443304197366No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG890.2655052056919543RNA PCR Primer, Index 41 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC870.25953879657528117No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA830.2476059783419349RNA PCR Primer, Index 41 (96% over 29bp)
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCG790.23567316010858863No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC790.23567316010858863No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC780.23268995555025207No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG760.22672354643357898No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG680.20285790996688643No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC640.1909250917335402No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTA640.1909250917335402No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG630.1879418871752036RNA PCR Primer, Index 41 (96% over 29bp)
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC630.1879418871752036No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTA620.18495868261686704No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACA610.18197547805853048No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC600.17899227350019392No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTA590.17600906894185733No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT580.17302586438352077RNA PCR Primer, Index 41 (95% over 24bp)
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA570.1700426598251842No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC570.1700426598251842No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATC570.1700426598251842No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC550.1640762507085111No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC550.1640762507085111No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATAC540.16109304615017453No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC540.16109304615017453No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCT530.15810984159183797No Hit
CCCATGTCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC510.15214343247516482No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC500.14916022791682826No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATG490.1461770233584917No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC490.1461770233584917No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTC480.1431938188001551No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG480.1431938188001551RNA PCR Primer, Index 41 (96% over 25bp)
GGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACA480.1431938188001551No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAA470.14021061424181858No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCC470.14021061424181858No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGA470.14021061424181858No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG470.14021061424181858No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACC460.13722740968348202No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC460.13722740968348202No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA440.13126100056680887No Hit
CGTCAGATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCG440.13126100056680887No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG440.13126100056680887RNA PCR Primer, Index 41 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC440.13126100056680887No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG430.1282777960084723RNA PCR Primer, Index 41 (96% over 29bp)
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT430.1282777960084723No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATG430.1282777960084723No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC420.12529459145013574No Hit
GATGTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC420.12529459145013574No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACA410.12231138689179917No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCG410.12231138689179917No Hit
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC400.1193281823334626No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATC400.1193281823334626No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATG400.1193281823334626No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGA390.11634497777512604No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACG390.11634497777512604No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA370.11037856865845291No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAG370.11037856865845291No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACA370.11037856865845291No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT360.10739536410011635RNA PCR Primer, Index 41 (95% over 22bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC360.10739536410011635No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGG360.10739536410011635No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG350.10441215954177978No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG350.10441215954177978No Hit
GAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGA350.10441215954177978No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATC350.10441215954177978No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA350.10441215954177978No Hit
GTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGA350.10441215954177978No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG350.10441215954177978No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGC350.10441215954177978No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG340.10142895498344322TruSeq Adapter, Index 3 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG340.10142895498344322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)