FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002296584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4294556
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA379400.8834440626691094No Hit
GTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCG193690.4510128637279383No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTT189940.4422808783958109No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA183150.42647016362110546No Hit
GTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG178450.4155260753381723No Hit
GACTGGGAACGACCGCCTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAA162010.3772450516421255No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCC146300.3406638544240662No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAG143390.3338878338063353No Hit
GGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCT140500.32715838377704237No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGGTCGTTCCCAGTCCG138600.3227341778754311No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGGTCGTTCCCAGTCCGTCCCCCGGCCGGCACG137960.3212439190454147No Hit
AAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC126990.2956999512871645No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAA126630.29486168069528024No Hit
TACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGGTCGTTCCCAGTCCGTCCCCCGGCCG110170.2565340864107954No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGGTCGTTCCCAGTCCGTCCCCC104530.2434011804712757No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAG104190.2426094804678295No Hit
GTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACG102190.2379524216240282No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGC101560.2364854480882308No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGA96670.22509893921513657No Hit
GCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGACCGCCTCGGC93820.2184626303627197No Hit
ATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG89990.20954436267684018No Hit
GGGAACGACCGCCTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCG87780.2043983126544397No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAAT84350.19641145673732047No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGGTCGTTC84250.1961786037951304No Hit
GCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT83860.19527047732058914No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG83850.19524719202637014No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGC82140.19126540671492No Hit
GAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTC81100.18884373611614333No Hit
GGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGT78770.1834182625631148No Hit
AGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGACCGCCTCGGCGGTCT77110.1795529037227597No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTCACG75560.17594368311881367No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAG70520.16420789483243436No Hit
GCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCG67520.1572223065667324No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTCACGCAATG66880.15573204773671598No Hit
GAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGT63760.1484670359403859No Hit
GTCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCG62920.1465110712259894No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTCACGCAATGTGATTT62660.1459056535762952No Hit
TCCCAGTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCC59880.13943234178341138No Hit
GTTCGACGCCCGGGGAAGACCGCCGAGGCGGTCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCT57660.1342630064667919No Hit
CACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTA55370.1289306740906394No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGAC55360.1289073887964204No Hit
GGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAG55290.12874439173688734No Hit
GTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACG53550.12469275054278021No Hit
TCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGA53490.12455303877746617No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCA53260.12401747701042902No Hit
GTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAA52960.12331891818385882No Hit
CCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGG52800.12294635347635471No Hit
GATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGGTCGTTCCCAG51140.11908099463599961No Hit
CTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGACCGCCTCG48880.11381851814250413No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGAC48850.11374866225984712No Hit
CATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACC48630.11323638578702898No Hit
GGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGGTCGTTCCCA47720.11111742401309938No Hit
GTACATGGGGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCC47380.11032572400965315No Hit
GTATCAACGCAGAGTACATGGGGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCC46140.10743834752649634No Hit
TCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGG45510.10597137399069893No Hit
GGGGACGGACTGGGAACGACCGCCTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACA43480.10124445926424058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)