FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026128
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGC285041.406821286710415No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGC139110.6865805121887659No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC124810.6160025427811076No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC123840.6112150861149938No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC99710.49212093214249053No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC97100.47923921884500886No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG86220.4255407358271541TruSeq Adapter, Index 4 (96% over 28bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAA55860.27569827770012556No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTC47650.23517763931992453No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAAC36180.17856719812371183No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG34200.16879486389803605No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG31350.15472862523986639No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAT30410.1500892342438385No Hit
GTCTAAACCCAGCTCACGTTCCCTTTTAACGGGTGAACAATCCAACACTTGGCGAATGCTGCTTCGCAATGATAG30370.14989181335039048No Hit
ATCCAACACTTGGCGAATGCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTA30300.1495463267868565No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTTTTAACGGGTGAACAATCCAACACT29490.1455485536945346No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG29310.14466015967401863RNA PCR Primer, Index 4 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG26650.13153167025972692RNA PCR Primer, Index 4 (96% over 28bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAA26390.13024843445231496No Hit
ATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG24260.1197357718762092TruSeq Adapter, Index 4 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAT22170.10942053019355144No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT20810.1027082198163196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)