FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227587
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC158190.7101406140366234No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC82610.3708497131649628No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC72740.3265416794046652No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC69580.3123559259413886No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG68020.3053528324595179TruSeq Adapter, Index 7 (96% over 28bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC57360.2574983603334011No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC53670.24093335075128378No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCATTTCCGGCCATCACAATGCTTTGTTTTA45980.20641169121565173No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCA42360.19016092300772092No Hit
GTGTCAACACCATTTCCGGCCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT36000.16160984958163252No Hit
GGATTGGCTCTGAGGGCTGGGTCTGGGAATCTGCTCCACGAACGCCCGCGCCTTCGGGCGGCTGCCCGACTGCCT31230.1401965445120662No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC26260.11788540694482415No Hit
TCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGCTGCTTGTTGCCT26260.11788540694482415No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC25790.11577550057528616No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG25120.11276776170807246No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG24940.1119597124601643RNA PCR Primer, Index 7 (96% over 28bp)
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGATAAGGATTGGCTCTGAGGGCTGGGTCTGGGA22720.10199377173596362No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGAC22720.10199377173596362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)