FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147198
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC377401.7576394910949062No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC193030.8989855616482504No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC158360.7375193158711959RNA PCR Primer, Index 32 (95% over 24bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC147860.6886183761348511RNA PCR Primer, Index 32 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG135500.6310549842166395RNA PCR Primer, Index 32 (96% over 33bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC119470.5563995495524865RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC113820.5300861867419772RNA PCR Primer, Index 32 (95% over 24bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA60580.2821351361169301No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC58590.2728672437288038RNA PCR Primer, Index 32 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG55120.25670664745403077RNA PCR Primer, Index 32 (96% over 33bp)
ACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG39840.18554413705675954RNA PCR Primer, Index 32 (96% over 33bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG37780.1759502384037243No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA36640.170640993518064No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATT34670.16146624577705457No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC32680.15219835338892826No Hit
ATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG32510.15140662388843507RNA PCR Primer, Index 32 (96% over 33bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA29970.13957725370459548No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATT28460.13254483284727353No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATG25380.11820055719127905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)