FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002296626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619476
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGC199711.2331766571409517No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG90280.5574642662194439Illumina PCR Primer Index 1 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGC86370.533320654335106No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC82570.509856274498665No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC78290.4834279729986736No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC64450.39796823169963613No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC61910.3822841462300151No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG35590.21976244167866643Illumina Paired End PCR Primer 2 (96% over 28bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGA32770.20234940190530767No Hit
ACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG30500.18833252237143372Illumina PCR Primer Index 1 (96% over 28bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC27620.17054899239013113No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC27310.16863479298242148No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG26420.16313918823125506No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAA23580.145602651721915No Hit
ATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG21780.13448794548360088Illumina PCR Primer Index 1 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC21090.1302273080922471No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAAC19290.119112601853933No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAA18730.1156546932464575No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG16420.10139082024062104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)