FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002296634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174904
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG197241.6787754573990725RNA PCR Primer, Index 17 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG70160.5971551718268046RNA PCR Primer, Index 17 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGC35900.3055568795407965No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG34120.29040670556913584RNA PCR Primer, Index 17 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA31420.26742610460088656No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG27100.23065714305168764RNA PCR Primer, Index 19 (96% over 26bp)
GAGCTGAGGTGAAGATCCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA19050.16214090683153687No Hit
GTATCAACGCAGAGTACATGGGGGATCTTCACCTCAGCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA15990.13609622573418764No Hit
GCAGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15610.13286191893124885No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG15060.12818068540067956No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT14550.12383990521778801No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG14160.12052048507792978RNA PCR Primer, Index 17 (96% over 28bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC13960.11881821833954094No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG13880.1181373116441854No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC13410.11413698480897162No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG13320.11337096477669664RNA PCR Primer, Index 17 (96% over 28bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT12900.10979620462608009No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC12190.1037531577047997No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA12070.10273179766176642No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG12040.10247645765100809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)