FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002296649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361577
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGC966332.8746329475719286No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC268820.7996841958402261No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGC231860.6897357995964394No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC217860.6480886798071263No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC197980.5889497697063015No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC166860.49637417200319967No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG106000.315328192690514TruSeq Adapter, Index 1 (96% over 28bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGA57990.17250831975587647No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGC57420.1708126870215973No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC50130.1491264367884478No Hit
CCTTAGAAGTGTGAGCAATCTTTAAGGCCCACTCTCCAGGATCGAACCCTGATCCTCTGTACCCGTTGTGACCAT45440.1351746516590279No Hit
ATCCTGGAGAGTGGGCCTTAAAGATTGCTCACACTTCTAAGGAAGGCAGCAGGCGCGTAACGTTCCCATTGGACA44270.13169414236234958No Hit
CTCCAGGATCGAACCCTGATCCTCTGTACCCGTTGTGACCATGAATGGACGTGGCGCTTTCATCGGGAGTTGATA42000.12494135936793951No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT41820.12440589639921976TruSeq Adapter, Index 1 (95% over 21bp)
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC40100.11928925025367558TruSeq Adapter, Index 1 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG36460.10846099910845415RNA PCR Primer, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)