FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002296681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797240
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG293383.6799458130550398RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG116191.4574030404896894RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG53390.6696854146806482RNA PCR Primer, Index 29 (96% over 30bp)
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG32820.41167026240529825No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA32620.40916160754603376No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA23180.2907530981887512No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA21310.2672971752546284No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG14700.184386132155938RNA PCR Primer, Index 29 (96% over 30bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG13690.17171742511665244RNA PCR Primer, Index 29 (96% over 30bp)
TATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGG12620.1582961216195876No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG10830.13584366062917064Illumina PCR Primer Index 10 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)