FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002296723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002296723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5029
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC320.6363094054483992No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC280.5567707297673493No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGA270.5368860608470869No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC260.5170013919268244No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC230.45734738516603696No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG220.4374627162457745No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCA220.4374627162457745No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.3976933784052496No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA190.37780870948498707No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAT180.3579240405647246No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA180.3579240405647246No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCA170.3380393716444621No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCA170.3380393716444621No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATC160.3181547027241996No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.3181547027241996No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAT150.2982700338039372No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCA150.2982700338039372No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGA150.2982700338039372No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC150.2982700338039372No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCAC140.27838536488367466No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG140.27838536488367466No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC140.27838536488367466No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG130.2585006959634122No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCC130.2585006959634122No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC130.2585006959634122No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAT120.23861602704314974No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCAT120.23861602704314974No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC120.23861602704314974No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCG120.23861602704314974No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCG120.23861602704314974No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC110.21873135812288724No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAT110.21873135812288724No Hit
GCCAAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTA110.21873135812288724No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCC110.21873135812288724No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC110.21873135812288724No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAT110.21873135812288724No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCG110.21873135812288724No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCAC100.1988466892026248No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCA100.1988466892026248No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAT100.1988466892026248No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC100.1988466892026248No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG100.1988466892026248TruSeq Adapter, Index 7 (96% over 28bp)
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGAC100.1988466892026248No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATC100.1988466892026248No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCA90.1789620202823623No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC90.1789620202823623No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCC90.1789620202823623No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGA90.1789620202823623No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC90.1789620202823623No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG80.1590773513620998No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCC80.1590773513620998No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGA80.1590773513620998No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC80.1590773513620998No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCAT80.1590773513620998No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATG80.1590773513620998No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAG80.1590773513620998No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTC70.13919268244183733No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC70.13919268244183733No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGAC70.13919268244183733No Hit
AGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGGTTCATC70.13919268244183733No Hit
TTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCG70.13919268244183733No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCG70.13919268244183733No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCG70.13919268244183733No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTC70.13919268244183733No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATC70.13919268244183733No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGA70.13919268244183733No Hit
TTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCT70.13919268244183733No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTC70.13919268244183733No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCG60.11930801352157487No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGA60.11930801352157487No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGA60.11930801352157487No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC60.11930801352157487No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCA60.11930801352157487No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC60.11930801352157487No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGA60.11930801352157487No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACC60.11930801352157487No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCG60.11930801352157487No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGAC60.11930801352157487No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC60.11930801352157487No Hit
CTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCA60.11930801352157487No Hit
TCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATC60.11930801352157487No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATC60.11930801352157487No Hit
GGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCA60.11930801352157487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)